Fig. 6: AP-1 loss leads to enhancer architecture changes and ECM gene dysregulation.

a Principal component analysis plot of the top 500 most variable genes in AP-1-depleted and negative control HUtSMCs. First two principal components are plotted. b Hierarchically clustered heat map of all differentially expressed genes in AP-1-depleted HUtSMCs. Gene expression levels relative to the mean expression are shown as row Z-scores. c Scatter plot of confidence scores for enriched gene ontologies associated with differentially expressed genes in AP-1-depleted HUtSMCs, with ontologies clustered by functional similarity in the semantic space. d Heat map of normalized H3K27Ac ChIP-seq reads at differentially acetylated regions in negative control vs. AP-1 depleted HUtSMCs. Signal for each replicate is shown. e Pie charts of H3K27Ac signal distribution at genomic loci in negative control HUtSMC (top left), AP-1-depleted HUtSMC (top right), and at differentially acetylated regions in HUtSMCs (bottom left). f Top identified transcription factor motifs (P value ranking, binomial) enriched at sites with differential H3K27Ac signal between negative control and AP-1-depleted HUtSMCs (top panel). Lower panel shows location of AP-1 motifs relative to peak center for all differentially acetylated regions.