Fig. 2: Sequence-independent recognition of Hs mt tRNAAla by mt AlaRS.
From: Relaxed sequence constraints favor mutational freedom in idiosyncratic metazoan mitochondrial tRNAs
a Hs mt tRNAAla mutants used in charging experiments with mt AlaRS. b–d Relative charging activities (kcat/Km) of mt AlaRS on mt tRNAAla variants (activity with wild-type mt tRNAAla is set to 1). e Sequence of Hs ct tRNAAla (left) and relative activities of chimeric tRNAAla variants. Domains are colored orange for mt tRNAAla and blue for ct tRNAAla. Error bars represent SEM of two independent experiments for each wild-type and mutant. Source data are provided as a Source Data file (see also Table 1).
