Table 2 Charging levels of mt tRNAAla constructs (related to Fig. 3).

From: Relaxed sequence constraints favor mutational freedom in idiosyncratic metazoan mitochondrial tRNAs

mt tRNAAla variant

tRNA charged [µM]

tRNA charged relative to WT [%]

H. sapiens WT

2.71 ± 0.03

100 ± 1.2

U62C

2.63 ± 0.03

97 ± 1.3

U68C

2.32 ± 0.08

86 ± 3.3

U62C/U68C

2.74 ± 0.05

101 ± 1.9

C6U

1.55 ± 0.05

57 ± 3.0

C6U + U62C

2.66 ± 0.08

98 ± 3.2

C6U + U68C

2.82 ± 0.07

104 ± 2.5

U7C

1.13 ± 0.05

42 ± 4.1

U7C + A66G

2.94 ± 0.07

109 ± 2.5

U7C + U62C

2.03 ± 0.08

75 ± 4.0

U7C + U62C/U68C

3.05 ± 0.08

113 ± 2.6

C28U

1.26 ± 0.02

46 ± 1.7

C28U + G42A

3.01 ± 0.11

111 ± 3.5

C28U + U62C/U68C

2.86 ± 0.12

106 ± 4.3

A31G

1.31 ± 0.05

48 ± 4.2

U39C

1.08 ± 0.02

40 ± 2.2

A31G + U39C

0.93 ± 0.03

34 ± 3.2

A31G + U62C/U68C

1.62 ± 0.07

60 ± 4.3

T. obscurus

3.19 ± 0.12

118 ± 3.7

C69U

1.41 ± 0.05

52 ± 3.5

C69U + U62C

2.53 ± 0.11

94 ± 4.3

C69U + U68C

2.93 ± 0.13

108 ± 4.4

C69U + U62C/U68C

2.83 ± 0.06

105 ± 2.1

A1G/U72C

1.07 ± 0.03

40 ± 3.2

A1G/U72C/U62C/U68C

2.55 ± 0.07

94 ± 2.8

C6U/C28U/A31G/C50U

0.97 ± 0.01

36 ± 0.9

P. coquereli

3.11 ± 0.07

115 ± 2.3

  1. Charging level of wild-type (WT) Hs mt tRNAAla is set to 100%. The data represent the mean of two to six independent experiments ± standard error.
  2. T. obscurus: Corresponds to Hs mt tRNAAla with mutations A31G + G26A/G27A/G54A/G57A/G59A/U62C/C65U.
  3. P. coquereli: Corresponds to Hs mt tRNAAla with mutations C6U/C28U/A31G/C50U + A1G/G5A/U19A/G26A/G27A/U39C/G42A/A53G/G58A/U61C.
  4. Source data are provided as a Source Data file.
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