Fig. 1: Visualizing the distribution of CD56 receptors in the plasma membrane of fixed whole 293T cells by 3D-LLS-dSTORM.
From: Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM
a Reconstructed volume rendering of single-molecule localizations shows considerable accumulation of CD56 receptors at cell–cell interfaces between the three cells. Representative 2D xy-projections of small areas of the basal (b) and apical (c) plasma membrane. Representative 2D projection perpendicular to the z-axis highlighting the increased CD56 density and the cell–cell interface (d). Note the lower spatial resolution in z-direction. e Localization densities (median ± MAD) measured at the basal (139 ± 76 localizations/µm2) and apical plasma membrane (361 ± 79 localizations/µm2), and at the cell–cell interface (1234 ± 146 localizations/µm2) for the shown cell. Boxplot of n = 7201 (basal), n = 854 (apical), and n = 628 (interface) sliding window data points. Boxplots show median (center line), 25th and 75th percentile (box), and 1.5× interquartile range (whiskers). Scale bars, 5 µm (a), 1 µm (b–d).
