Fig. 2: Single-particle tracking in living cells by LLSM.
From: Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM
a Trajectories of CD56 molecules in the plasma membrane of 293T cells measured at 37 °C. b Astigmatic detection enables tracking of CD56 receptors in 3D. c The ensemble mean square displacement for each dimension r\(\in\)(x, y, z) for all trajectories with (cross symbols) and without (plus symbols) actin inhibitor CK666. n = 7 different cells were tracked for each condition. Each cell yielded an ensemble of >100 trajectories. d Ensemble mean square displacement zoomed to the part from which diffusion constants were determined by fitting a linear function. In addition to the LLS microscopy data (n = 7 tracking experiments examined from different cells of one biological sample), 2D-TIRF data are shown for 293T cells (n = 6 tracking experiments examined from different cells of one biological sample) adhered on cleaned coverslips (circle symbols).
