Fig. 5: Mono- and multi-functionalization of proteins using 3Br-5MPs. | Nature Communications

Fig. 5: Mono- and multi-functionalization of proteins using 3Br-5MPs.

From: Cysteine-specific protein multi-functionalization and disulfide bridging using 3-bromo-5-methylene pyrrolones

Fig. 5

a A schematic representation of mono- and multi-functionalization of H3-V35C (20). b Fifteen percent SDS-PAGE analysis of the stability of the doubly modified product 23 upon incubation with 1 mM GSH in HEPES buffers (pH 7.5). c Fifteen percent SDS-PAGE analysis of the stability of the reduced doubly modified product 25 upon incubation with 1 mM GSH in HEPES buffers (pH 7.5). Source data are provided as a Source Data file.

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