Fig. 7: Disulfide bridging bioconjugation of an antibody fragment with 3Br-5MP 3e. | Nature Communications

Fig. 7: Disulfide bridging bioconjugation of an antibody fragment with 3Br-5MP 3e.

From: Cysteine-specific protein multi-functionalization and disulfide bridging using 3-bromo-5-methylene pyrrolones

Fig. 7

a Strategy for disulfide bridging bioconjugation of the Fab fragment of goat anti-human IgG via 3Br-5MP. b SDS-PAGE gel analysis of disulfide bridging bioconjugation of the Fab fragment, with fluorescence imaging (top gels) and Coomassie staining (bottom gels). c Biological activity of bridged bioconjugate 33. Trastuzumab binding to HER2 on the surface of SK-BR-3 cells was immunodetected with 33 (second row). Goat anti-human antibody-Alexa488 conjugate (Ab-AF488) at an equivalent concentration was used as a positive control (third row) and an experiment in the absence of trastuzumab was carried out as a negative control (first row). Scale bar, 50 μm.

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