Fig. 4: Subclustering reveals additional heterogeneity in cancer stem cell-like and basal cells.
From: Tracing tumorigenesis in a solid tumor model at single-cell resolution
a Subclustering of the tumor-specific epithelial subset identifies two cancer stem cell-like (‘CSC 1’ and ‘CSC 2’) and two basal cell (‘basal normal’ and ‘basal tumor’) subpopulations. b Expression of several genes in the subclustered tumor-specific tSNE representation as indicated by a color scale ranging from gray (no expression) to dark blue (high expression) with regions of high expression highlighted. c Gene set enrichment analysis on differentially expressed genes between CSC-like or basal subclusters, respectively. Shown are log2 fold changes for all genes per pathway expressed in at least 5% of cells, with selected genes highlighted. Orange dots indicate the pathway mean, dashed lines the cutoff set on the pathway mean.
