Fig. 3: Comparison of 4WJ-X and 4WJ-X-24 PTXs nanoparticles by single-particle cryo-EM.

a Representative motion-corrected cryo-EM micrographs of 4WJ-X and b 4WJ-X-24 PTXs (scale bar: 500 Å). c Reference-free 2D class averages of 4WJ-X and d 4WJ-X-24 PTXs computed in Relion. e Gold standard Fourier shell correlation (FSC) plots to measure resolution at FSC = 0.143 for the 3D reconstruction of 4WJ-X and f 4WJ-X-24 PTXs. g 3D reconstructed cryo-EM maps of 4WJ-X and 4WJ-24 PTXs in four views, with a Supplementary Movie 1. A 3D design model is shown. Source data are provided as a Source Data file.