Fig. 4: SeeNet retains nucleic acids, proteins, and fluorescence of endogenous probes.

a Maximum projection of volumetric images of the cerebral hemisphere. The vasculature was cast using FITC–Dex–GMA (green). After delipidation with SDC, nucleic acids were labeled using PI (magenta). Then, the sample was RI-matched using ScaleCUBIC-2. Scale bar = 1 mm. b An optical section of a boxed region in panel a at Z = 200 μm. PI fluorescence was uniformly retained across the section. Scale bar = 100 μm. c The normalized median intensities of PI fluorescence were plotted as a function of the distance from the nearest vessel. The intensities were almost unchanged depending on the distance. n = 7 hemispheres from seven mice. d–f The same as panels a–c showing the immunohistochemical signal of anti-Iba-1 (magenta). g–i The same as panels a–c showing RITC–Dex–GMA (magenta) and Arc-dVenus (green). Error bars represent standard deviations. Source data are provided as a Source Data file.