Table 1 Antibodies used for western blots.
From: AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy
Antibodies | Used dilution | Vendor |
|---|---|---|
rabbit polyclonal anti-pan-14-3-3 (K-19) | 1:1000 | Santa Cruz, sc-629; RRID: AB_2273154 |
rabbit monoclonal anti-ACC (C83B10) | 1:1000 | CST, 3676; RRID: AB_2219397 |
rabbit monoclonal anti-phospho-ACC (Ser79) (D7D11) | 1:1000 | CST, 11818; RRID: AB_2687505 |
mouse monoclonal anti-actin (C4) | 1:200 | MP Biomedicals 0869100; RRID: AB_2335304 |
rabbit monoclonal anti-AMPK-α (D5A2) | 1:1000 | CST, 5831; RRID: AB_10622186 |
rabbit monoclonal anti-phospho-AMPK-α (Thr172) (40H9) | 1:1000 | CST, 2535; RRID: AB_331250 |
rabbit monoclonal anti-AMPK-β1/2 (57C12) | 1:1000 | CST 4150; RRID: AB_560860 |
rabbit polyclonal anti-CDK16 (PCTAIRE-1) (C-16) | 1:1000 | Santa Cruz sc-174; RRID: AB_2158996 |
rabbit polyclonal anti-CDK16 (PCTAIRE-1) | 1:1000 | Sigma-Aldrich, HPA001366; RRID: AB_1079584 |
sheep polyclonal anti-phospho-CDK16 (Ser12) | 0,1 µg/ml | This paper (MRC-PPU, University Dundee) |
rabbit polyclonal anti-phospho-CDK16 (Ser65) | 0,1 µg/ml | This paper (YenZym) |
rabbit polyclonal anti-phospho-CDK16 (Ser119) | 0,1 µg/ml | This paper (MRC-PPU, University Dundee) |
rabbit polyclonal anti-phospho-CDK16 (Ser153) | 0,1 µg/ml | This paper (YenZym) |
rabbit polyclonal anti-phospho-CDK16 (Ser155) | 0,1 µg/ml | This paper (YenZym) |
rabbit polyclonal anti-phospho-CDK16 (Ser461) | 0,1 µg/ml | This paper (YenZym) |
rabbit polyclonal anti-Cyclin Y | 1:1000 | Bethyl, A302-376A; RRID: AB_1907259 |
mouse monoclonal anti-Cyclin Y (2C9E3) | 1:2000 | Proteintech, 66865-1-Ig |
rabbit polyclonal anti-phospho-Cyclin Y (Ser326) | 0,25 µg/ml | Shehata et al., 201515 |
rabbit polyclonal anti-phospho-Cyclin Y (Ser336) | 0,25 µg/ml | Shehata et al., 201515 |
rabbit polyclonal anti-DYKDDDDK tag | 1:1000 | CST 2368; RRID: AB_2217020 |
mouse monoclonal anti-FLAG (M2) | 1:5000 | Sigma-Aldrich, F3165; RRID: AB_259529 |
mouse monoclonal anti-GAPDH (4G5) | 1:500 | Bio-Rad, MCA4740; RRID: AB_2107457 |
mouse monoclonal anti-GFP (9F9.F9) | 1:2000 | Rockland, 600-301-215; RRID: AB_218216 |
mouse monoclonal anti-HA | 1:1000 | Covance, MMS-101R; RRID: AB_10064220 |
rabbit polyclonal anti-LC3B | 1:1000 | CST, 2775; RRID: AB_915950 |
guinea pig polyclonal anti-p62/SQSTM1 | 1:1000 | Progen, GP62-C; RRID: AB_1542690 |
mouse monoclonal anti-α-tubulin (B-5-1-2) | 1:5000 | Sigma-Aldrich, T5168; RRID: AB_477579 |
goat polyclonal anti-guinea pig IgG (H + L) HRP-conjugated | 1:10,000 | Santa Cruz, sc-2438; RRID: AB_650492 |
AffiniPure rat polyclonal anti-mouse IgG (H + L) HRP-conjugated | 1:10,000 | Jackson ImmunoResearch, 415-035-166; RRID: AB_2340269 |
AffiniPure goat polyclonal anti-rabbit IgG (H + L) HRP-conjugated | 1:10,000 | Jackson ImmunoResearch, 111-035-144; RRID: AB_2307391 |
rabbit monoclonal anti-ULK1 (D8H5) | 1:1000 | CST, 8054; RRID: AB_11178668 |
rabbit monoclonal anti-phospho-ULK1 (S757) (D7O6U) | 1:1000 | CST, 14202; RRID: AB_2665508 |
rabbit monoclonal anti-Beclin1 (D40C5) | 1:1000 | CST, 3495; RRID: AB_1903911 |
rabbit monoclonal anti-Raptor (24C12) | 1:1000 | CST, 2250; RRID: AB_561245 |
rabbit polyclonal anti-phospho-Raptor (S792) (24C12) | 1:1000 | CST, 2083; RRID: AB_2249475 |
