Table 2 Antibodies used for immunofluorescence staining.
From: AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy
Antibodies | Used dilution | Vendor |
|---|---|---|
rabbit monoclonal anti-AMPK-β1/2 (57C12) | 1:50 | CST 4150; RRID: AB_560860 |
mouse monoclonal anti-Cyclin Y (2C9E3) | 1:50 | Proteintech, 66865-1-Ig |
mouse monoclonal anti-HA | 1:200 | Covance, MMS-101R; RRID: AB_10064220 |
mouse monoclonal anti-LC3 (4E12) | 1:50 | MBL, M152-3; RRID: AB_1279144 |
goat polyclonal anti-mouse IgG (H + L) Alexa Fluor 488 | 1:1000 | Thermo Fisher Scientific, A-11001; RRID: AB_2534069 |
goat polyclonal anti-mouse IgG (H + L) Alexa Fluor 555 | 1:1000 | Thermo Fisher Scientific, A-21422; RRID: AB_2535844 |
goat polyclonal anti-mouse IgG (H + L) Alexa Fluor 633 | 1:1000 | Thermo Fisher Scientific, A-21050; RRID: AB_2535718 |
mouse monoclonal anti-WIPI2 (2A2) | 1:500 | Bio-Rad, MCA5780GA; RRID: AB_10845951 |
