Fig. 2: Quantitative evaluation of native inducible systems.
From: A genome-wide approach for identification and characterisation of metabolite-inducible systems
a Chemical structures of the proposed primary effector molecules: β-alanine (1), formic acid (2), xanthine (3), phenylglyoxylic acid (4), salicylic acid (5), benzoic acid (6), tartaric acid (7), sulfoacetic acid (8), L-kynurenine (9), 3-hydroxypropionic acid (10), L-phenylalanine (11), γ-aminobutyric acid (12), L-tyrosine (13), cyclohexanecarboxylic acid (14), L-glutamine (15) and 3,4-dihydroxybenzoic acid (16). b Summary of the identified inducible systems, including the inducible promoter, TR name and corresponding ligand. c Single time-point RFP fluorescence measurements (arbitrary units) of C. necator H16 carrying the transcription factor-based inducible gene expression systems composed of TR and inducible promoter in the same order as listed in b. The plasmids harbouring the individual system–reporter constructs are indicated. d Single time-point RFP fluorescence measurements of C. necator H16 carrying the inducible ‘promoter only’ implementations in the same order as listed in b. The plasmids harbouring the individual promoter–reporter gene constructs are indicated. Fluorescence output was determined in the absence of inducer (light magenta) and 6 h after extracellular supplementation with the corresponding effector to a final concentration of 5 mM (dark magenta). Data are mean ± SD, n = 3, *p < 0.05, **p < 0.001, ***p ≤ 0.0001, unpaired two-tailed t-test. Source data are provided as a Source Data file.
