Fig. 6: Orthogonality of inducible systems.
From: A genome-wide approach for identification and characterisation of metabolite-inducible systems
a Cross-reactivity of a set of 21 inducible systems and their corresponding primary inducers. The heat map illustrates induction of reporter gene expression in the presence of the metabolite (in %) relative to the induction mediated by the corresponding primary effector. Measurements were taken 6 h after supplementation with inducer at a final concentration of 5 mM. Data are mean, n = 4. Source data are provided as a Source Data file. b Fluorescence output of individual cells of C. necator carrying pEH263 measured by flow cytometry. The vector pEH263 contains the 3,4-dihydroxybenzoate- and salicylate-inducible systems controlling expression of rfp and egfp, respectively. Fluorescence was determined in the absence of inducer (orange), in the presence of 3,4-dihydroxybenzoate (blue), salicylate (green), and both 3,4-dihydroxybenzoate and salicylate (purple). Effector concentrations correspond to their respective Km’s and fluorescence was measured 2 h after inducer addition.
