Fig. 1: Plant temperature varies with plant region and virus infection status.

a Thermal images of wheat plants in field, Rock Springs Experimental Station in central PA; upper regions (flag leaves) were significantly warmer than stems (Student’s t test = 47.41, df = 17, P ≤ 0.000; n = 20 plants). Thermal images in a rainbow scale, min. temperature in blue = 21.7 °C, max. temperature in white: 25.7 °C. b–e Virus effects on plant temperature in controlled laboratory experiments. b–d Thermal imaging of wheat plants in the laboratory experiments. Dry and wet control plants were used as a reference for 0% and 100% transpiration, respectively; untouched control received no manipulation. e Temperature of plants infected with BYDV strains, nonparametric ANOVA, Kruskal–Wallis (boxplots display median line, interquartile range (IQR) boxes, 1.5 × IQR whiskers; n = 6 plants per treatment). Thermal images in a rainbow scale, min. temperature in blue = 25.2 °C, max. temperature in white: 32 °C. Source data are provided as a Source data file. [Image by Porras and Rajotte].