Fig. 2: Molecular characterization of the carotenoid cassette at Target B. | Nature Communications

Fig. 2: Molecular characterization of the carotenoid cassette at Target B.

From: Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

Fig. 2: Molecular characterization of the carotenoid cassette at Target B.The alternative text for this image may have been generated using AI.

a Diagrams showing the genomic region near Target B in Kitaake rice and the donor DNA. Gray lines represent plasmid backbone DNA while black lines represent Kitaake genomic DNA. The vertical green triangles mark the positions of the guide RNA B target sites. b Diagram of the inserted carotenoid cassette at Target B in T0 plants #11, 16, 17, 24, 28, 48, and 50. The junction sequences in all the seven plants are identical, as shown in the diagram. For convenience, only the sequencing chromatograms for T0 #48 are shown. The protospacer adjacent motif (PAM) of the original guide RNA B targets are highlighted in yellow.

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