Fig. 4: ATML1 binds to the promoters of MIR171C and MIR170. | Nature Communications

Fig. 4: ATML1 binds to the promoters of MIR171C and MIR170.

From: A signal cascade originated from epidermis defines apical-basal patterning of Arabidopsis shoot apical meristems

Fig. 4

a Schematic structure of the MIR171C promoter. The expanded diagram shows the sequences of putative L1 boxes on the promoter. Probes indicate the DNA fragments used in the Y1H assay. +1 indicates the transcription start site. b The full-length promoter of MIR171C interacts with ATML1 protein in Y1H. Y-axis: the relative activity of the pMIR171C::lacZ. All the numbers are normalized to the average value of the empty vector control. Bars: mean ± SE (n = 5 biological replicates). ***P < 0.001 (Student’s two-tailed t-test). c The MIR171C-F1 fragment from the MIR171C promote interacts with the ATML1 protein in Y1H. Y-axis: the relative activity of the pMIR171C-F1::lacZ, pMIR171C-F2::lacZ and pMIR171C-F3::lacZ reporters. All the numbers are normalized to the average value of the empty vector control. Bars: mean ± SE (n = 5 biological replicates). ***P < 0.001 (Student’s two-tailed t-test). d Schematic structure of the MIR170 promoter. The expanded diagram shows the sequences of putative L1 boxes on the promoter. Probes indicate the DNA fragments used in the Y1H assay. +1 indicates the transcription start site. e The full-length promoter of MIR170 interacts with ATML1 protein in Y1H. Y axis: the relative activity of the pMIR170::lacZ reporter. All the numbers are normalized to the average value of the empty vector control. Bars: mean ± SE (n = 5 biological replicates). ***P < 0.001 (Student’s two-tailed t-test). f The MIR170-F2 fragment from the MIR170 promoter interacts with the ATML1 protein in Y1H. Y-axis: the relative activity of each lacZ reporter. All the numbers are normalized to the average value of the empty vector control. Bars: mean ± SE (n = 5 biological replicates). ***P < 0.001 (Student’s two-tailed t-test). Source data underlying Fig. 4b, c and Fig. 4e, f are provided as a Source Data file.

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