Fig. 1: Brassica tournefortii is highly sensitive to KAR1, the major karrikin analogue isolated from plant-derived smoke.
From: Divergent receptor proteins confer responses to different karrikins in two ephemeral weeds
a Germination responses of B. tournefortii seed to KAR1 and KAR2. Data are cumulative germination after 11 days (mean ± SE; n = 3 biological replicates per treatment, ≥35 seeds per replicate). b Levels of karrikin-responsive transcripts BtDLK2 and BtSTH7 in B. tournefortii seed. Seeds were imbibed for 24 h in the dark supplemented with KAR1 and KAR2. Transcripts were normalised to BtCACS reference transcripts. Data are means ± SE, n = 3 biological replicates, ≥50 seeds per replicate. c, d Hypocotyl elongation responses of B. tournefortii seedlings grown for 4 days under continuous red light on water-saturated glass fibre filter paper containing 0.1% acetone (mock), KAR1 or KAR2. Data are means ± 95% CI of n = 18–24 seedlings. Scale bar: 10 mm. e Levels of BtDLK2 transcripts in B. tournefortii seedlings grown under the same conditions as for d. Data are means ± SE, n = 3 biological replicates, ≥20 seedlings per replicate. In all panels, asterisks denote significance levels (ANOVA) between indicated conditions: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.
