Table 1 4i medium composition.
From: A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons
Component | Final concentration | Supplier |
|---|---|---|
Knockout DMEM | – | GIBCO |
Knockout serum replacement (KSR) | 20% | GIBCO |
l-glutamine | 2 mM | GIBCO |
Nonessential amino acids | 0.1 mM | GIBCO |
2-mercaptoethanol | 0.1 mM | GIBCO |
Penicillin–streptomycin | 100 U/ml (penicillin) | GIBCO |
0.1 mg/ml (streptomycin) | ||
Human LIF | 20 ng/ml | Stem Cell Institute (SCI) |
bFGF | 8 ng/ml | SCI |
TGF-β1 | 1 ng/ml | Peprotech |
CHIR99021 (CH) | 3 μM | Miltenyi Biotec |
PD0325901 (PD) | 1 μM | Miltenyi Biotec |
SB203580 (SB) | 5 μM | TOCRIS bioscience |
SP600125 (SP) | 5 μM | TOCRIS bioscience |
