Table 3 Buffer recipes for ChIP.
From: A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons
Buffer A | 1x |
 Buffer A stock, µl | 250 |
 H2O, µl | 750 |
 DTT, µl | 0.5 |
 PIC, µl | 5 |
Buffer B | 1x |
 Buffer B stock, µl | 275 |
 H2O, µl | 825 |
 DTT, µl | 0.55 |
ChIP buffer 1 | 1x |
 ChIP buffer stock, µl | 10 |
 H2O, µl | 90 |
 PIC, µl | 0.5 |
ChIP buffer 2 | 1x |
 ChIP buffer stock, µl | 40 |
 H2O, µl | 360 |
 PIC, µl | 2 |
 tRNA (Sigma) [10 mg/ml], µl | 2 |
Elution | 1x |
 Elute buffer, µl | 25 |
 H2O, µl | 25 |
Blocking buffer | 1x |
 ChIP buffer (CST), µl | 150 |
 H2O, µl | 1110 |
 PIC (Roche), µl | 75 |
 BSA (Sigma) (10%), µl | 150 |
 tRNA (Sigma) [10 mg/ml], µl | 15 |
Low salt | 1x |
 ChIP buffer (CST), µl | 300 |
 H2O, µl | 2700 |
High salt | 1x |
 ChIP buffer (CST), µl | 100 |
 H2O, µl | 900 |
 NaCl, µl | 70 |
LiCl Wash buffer | For 250 mL |
 20 mM Tris-HCl pH 8.0, ml | 5 ml of 1M |
 2 mM EDTA, ml | 1 ml of 0.5 M |
 250 mM LiCl, g | 2.65 g |
 1% NP-40, ml | 2.5 ml |
 1% Deoxycholate, g | 2.5 g |
 H2O, ml | 241.5 ml |
