Fig. 1: Overview of phage-assisted active site-directed ligand evolution.
From: An amber obligate active site-directed ligand evolution technique for phage display
a A diagram that illustrates the interaction between a protein target and a ligand. b The genetic incorporation of a ligand-fused ncAA into phage-displayed peptides for active site-directed binding to a protein target that is followed by stringent wash and elution to select high-affinity and selective phages. c ncAAs that have been genetically incorporated and can potentially serve as ligands for epigenetic regulators (HAT histone acetyltransferase, KDM protein lysine demethylase, KMT protein lysine methyltransferase, Bromo, YEATS, chromo, Tudor, and PhD are epigenetic reader domains).
