Fig. 3: Characterization of the amber-obligate phage library generated from superinfection-immunity-based selection.
From: An amber obligate active site-directed ligand evolution technique for phage display
a During two rounds of superinfection-immunity-based selection, the percentage of the population that was susceptible to superinfection by the CM13 helper phage (e.g., clones that contained amber codons) was monitored by growth on kanamycin. The increase in the number of cells that were susceptible to superinfection between rounds 1 and 2 is indicative of the successful selection of library clones containing amber codons. b The occurrence percentages of TAG codons across the seven randomized positions in the enriched amber- obligate phage library. c A heat map showing the occurrence abundance (amount at position/total sequences) of each amino acid or the TAG codon across all library positions. d A heat map showing deviation from theoretical randomization for each amino acid or the TAG codon at all library positions. Deviation is calculated by (observed abundance-expected abundance)/expected abundance.
