Fig. 4: The genetic incorporation of ncAAs into a phage-displayed peptide library.
From: An amber obligate active site-directed ligand evolution technique for phage display
a A schematic representation of the three-plasmid system used to incorporate ncAAs into the peptide library. b The structures of phenylalanine derivatives 1–4. c Phage yield in the presence and absence (NA) of 4 mM ncAAs 1–4. The yield is displayed in millions of colony-forming units per milliliter of culture media. Error bars represent one standard deviation of the mean of three independent experiments (n = 3). d The structures of two post-translationally modified lysines, 5 and 6. e Phage yield in the presence and absence (NA) of 5 and 6. The yield is displayed in millions or hundreds of thousands of colony-forming units for compounds 5 and 6, respectively. Error bars represent one standard deviation of the mean of four independent experiments (n = 4). Data for the incorporation of ncAAs are available in the Source Data File.
