Fig. 6: Binding and inhibition of human sirtuins by synthetic ligands derived from selected peptides.
From: An amber obligate active site-directed ligand evolution technique for phage display
a Fluorescent polarization assay of SIRT2 binding to FITC-conjugated S2P03 and its deacylated counterpart S2P03-K. Values are reported as the mean of three independent experiments (n = 3). b Inhibition of human SIRT1–3 by S2P04-tBuK (left) and TB (right). Values are reported as the mean of two independent experiments (n = 2). c Inhibition of SIRT2 by four tMyK-containing peptides. Values are reported as the mean of two independent experiments (n = 2). d Interactions between S2P03-tBuK and SIRT2 predicted by molecular dynamic simulation. Data associated with all inhibition and binding curves can be found in the Source Data File.
