Table 1 Cryo-EM statistics.
FliPQR, P. savastanoi (EMD-10095) (PDB 6S3R) | FliPQR, V. mimicus (EMD-10096) (PDB 6S3S) | FliPQR–FlhB, V. mimicus (EMD-10093) (PDB 6S3L) | FliPQR–FlhB amphipol, V. mimicus (EMD-10653) | |
|---|---|---|---|---|
Data collection and processing | ||||
Magnification | 165,000 | 165,000 | 165,000 | 165,000 |
Voltage (kV) | 300 | 300 | 300 | 300 |
Electron exposure (e−/Å2) | 48 | 48 | 48 | 48 |
Defocus range (μm) | 0.5–4 | 0.5–4 | 0.5–4 | 0.5–4 |
Pixel size (Å) | 0.822 | 0.822 | 0.822 | 0.822 |
Symmetry imposed | C1 | C1 | C1 | C1 |
Initial particle images (no.) | 503,177 | 1,050,955 | 1,386,230 | 677,403 |
Final particle images (no.) | 97,987 | 243,489 | 162,408 | 137,136 |
Map resolution (Å) | 3.5 | 4.1 | 3.2 | 4.0 |
FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
Refinement | ||||
Initial model used | EMDB-4173 | EMDB-4173 | EMDB-4173 | |
Model resolution (Å) | 3.5 | 4.1 | 3.2 | |
FSC threshold | 0.143 | 0.143 | 0.143 | |
Map sharpening B factor (Å2) | −101 | −214 | –97 | |
Model composition | ||||
Non-hydrogen atoms | 12,855 | 12,321 | 13,849 | |
Protein residues | 1669 | 1569 | 1768 | |
Ligands | 0 | 0 | 0 | |
B factors (Å2) | ||||
Protein | 36.94 | 101.85 | 43.55 | |
Ligand | NA | NA | NA | |
R.m.s. deviations | ||||
Bond lengths (Å) | 0.0058 | 0.0066 | 0.01 | |
Bond angles (°) | 0.88 | 0.87 | 0.93 | |
Validation | ||||
MolProbity score | 2.54 | 2.25 | 2.45 | |
Clashscore | 18 | 18.28 | 15.56 | |
Poor rotamers (%) | 2.22 | 0.07 | 2.72 | |
Ramachandran plot | ||||
Favoured (%) | 90.82 | 91.86 | 93.64 | |
Allowed (%) | 8.39 | 7.76 | 5.84 | |
Disallowed (%) | 0.79 | 0.39 | 0.52 | |
