Fig. 4: Extraction into an aqueous environment.
From: Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins
a Schematic of the process of extraction from water-in-oil to an all aqueous (buffered saline) environment. b Microscopy images of (i) unextracted (500 µM) and (ii) extracted xPOP(V)-25% porous microparticles and (iii) unextracted and (iv) extracted xPOP shells formed from mixtures of ELP(V) (1 mM) + xPOP (V1A4)-12.5% (100 µM) mixtures. The inset in iv demonstrates that hollow shell networks created with faster heating rates can also be extracted. c SEM of a xPOP(V)-25% microparticle showing the interconnected coacervate architecture that comprises the networked particle. d Cryo-SEM images of (i) the outer surface and (ii) a fractured xPOP (V1A4)-12.5% shell. The walls of each shell range from ~200–400 nm thick are composed of tightly packed nano-coacervates of xPOP.
