Fig. 6: Self-association of the ANK and CC domains of YrU1.

a, b Self-association of ANK (a) and CC (b) domains of YrU1 in firefly luciferase complementation imaging (LUC) assay. c, d Self-association of ANK (c) and CC (d) domains of YrU1 in yeast two-hybrid assays. The coding sequences of YrU1-ANK and YrU1-CC were fused to the Gal4 transactivation domain (AD) or the Gal4 DNA-binding domain (BD). Eight pairs of constructs were co-transformed into AH109. A 10-μL suspension (OD₆₀₀ = 0.5) of each co-transformant was dropped onto synthetic dropout (SD) medium lacking Leu and Trp and SD medium lacking Ade, His, Leu and Trp. Photographs were taken after 3 d of incubation. e, f The ANK (e) and CC (f) domains of YrU1 can self-associate in planta. Shown are immunoblots with anti-GFP and anti-MYC antibodies of total proteins extracted at 48 h post inoculation (hpi) from N. benthamiana leaves transiently expressing YrU1-ANK-MYC, YrU1-ANK-GFP, YrU1-CC-MYC or YrU1-CC-GFP and immunoprecipitated with anti-GFP. All experiments were repeated at least two times with similar results.