Fig. 6: EPS production in situ mediates cell aggregation and microcolony structuring. | Nature Communications

Fig. 6: EPS production in situ mediates cell aggregation and microcolony structuring.

From: Dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization

Fig. 6

Z-projection (max intensity) of the CLSM images stack showing S. mutans growth on HAD in (a) 0.5 %-w/v fructose + 0.5 %-w/v glucose (0.5% Fru + 0.5% Glu) for bacteria (green), in (b) 1 %-w/v sucrose (1% Suc) for bacteria (green) and extracellular polymeric matrix (red), and in (c) 1 %-w/v sucrose with EPS-degrading enzymes (1% Suc, Dex/Mut) for bacteria (green) and extracellular polymeric matrix (red) over time. Scale bar indicates 2 μm. d Morphological analysis of the biofilm-forming elements formed over time, evaluated by the ratio between the volume and the convex hull volume (Volume/qhull volume). e Curves showing volume growth and surface area occupation of S. mutans growing on HAD in different conditions (n = 3). Source data are provided as a Source Data file.

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