Fig. 5: Effect of the passport sequence tagging on the ER-to-Golgi transport of EPO. | Nature Communications

Fig. 5: Effect of the passport sequence tagging on the ER-to-Golgi transport of EPO.

From: Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor

Fig. 5

a RUSH imaging of EPO and EPO-motif expressed in HCT116. Before the addition of biotin (time 0), both EPO and EPO-motif exhibited a typical ER localization. At 30 min after biotin addition, EPO was localized in the Golgi to varying extents depending on the C-terminal motif. GM130 was used as a cis-Golgi marker. Bar, 10 µm. Consistent data were obtained from two independent experiments. b Quantification of the trafficking efficiency to the Golgi complex in HCT116 cells. Colocalized fractions of EPO and EPO-motif with GM130 were quantified based on Manders’ M1 coefficient32. Data are means of at least nine cells. Error bars show SEM. Statistical significance (p) was assessed by Wilcoxon’s and Mann–Whitney tests with two tailed.

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