Fig. 1: CryoEM analysis of the AdhE spirosomes in their compact and extended forms. | Nature Communications

Fig. 1: CryoEM analysis of the AdhE spirosomes in their compact and extended forms.

From: Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation

Fig. 1

a Spirosomes were incubated with different cofactors as indicated in the panel. The condition apo corresponds to the spirosomes as they were purified from E. coli. For each condition, five representative 2D classes are displayed. b CryoEM map of the extended spirosomes (incubated with NAD+, Fe2+, and CoA) obtained by single-particle analysis. An AdhE dimer is colored with each AdhE protomer in blue and light blue, respectively. Adjacent ADH domains are colored in orange and pink. c CryoEM map of the compact spirosomes (incubated with NADH and Fe2+) obtained by single-particle analysis. The same color codes as in b are used. d CryoEM map of the extended spirosomes obtained by helical reconstruction. Representative 2D classes of the helical segments are displayed and the refined helical parameters are indicated. The same color codes as in b are used. e CryoEM map of the compact spirosomes obtained by helical reconstruction. Representative 2D classes of the helical segments are displayed and the refined helical parameters are indicated. The same color code as in b are used.

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