Fig. 1: Validated expression of Grp and Cre in GrpCre-KI sensory neurons. | Nature Communications

Fig. 1: Validated expression of Grp and Cre in GrpCre-KI sensory neurons.

From: Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors

Fig. 1

a Schematic of targeting strategy to knock in eGFP-Cre cassette in Grp allele to generate GrpCre-KI mice. b Gel electrophoresis of genotyping PCR from Grp Cre/wt, wt/wt, and Cre/Cre samples. c eGFP IHC from DRG section of an adult GrpCre-KI mouse. d Image of eGFP epifluorescent signal (no GFP antibody) in GrpCre-KI DRG. e–g GRP IHC (e) and eYFP fluorescent image (f) from DRG section of an adult GrpCre-KI mouse that received AAV5- EF1α-DIO-eYFP injection into the DRG for 3 weeks. Scale bar, 20 μm. h and i IHC of GRP (h) and SP (i) in cervical spinal sections from WT mice following i.d. injections of CQ (200 μg). Scale bar, 100 μm. Data are presented as mean ± s.e.m., n = 3 mice and 9 sections. Source data are provided as a Source Data file.

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