Fig. 2: GrptdTom recapitulates Grp expression in DRG and the spinal cord. | Nature Communications

Fig. 2: GrptdTom recapitulates Grp expression in DRG and the spinal cord.

From: Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors

Fig. 2

a Schematic of GrpCre-KI mating with Ai14 reporter line to produce GrptdTom mice. b IHC images of eGFP and tdTomato epifluorescence in GrptdTom DRG. Scale bar, 50 μm. c Percentage of eGFP+ neurons co-expressing tdTomato or of tdTomato+ neurons co-expressing eGFP. n = 3 mice and 10 sections. d–f IHC images of tdTomato epifluorescence (d) and GRP (e) in GrptdTom DRG. Scale bar, 20 μm. g–i IHC of tdTomato epifluorescence (g) and GRP (h) in GrptdTom spinal cord. Scale bars, 50 µm. j IHC image of tdTomato, CGRP and DAPI in GrptdTom nape skin. Arrows indicate apparent GrptdTom fibers co-expressing CGRP. Arrowheads indicate autofluorescent hair shafts. Dashed line indicates epidermal/dermal boundary. k IHC images of tdTomato, CGRP, and DAPI in glabrous paw skin with stratum basilis (SB), stratum spinosum (SS), stratum granulosum (SG), and stratum corneum (SC) epidermal layers marked by dashed lines. Arrows indicate GrptdTom fibers with ‘S’ or ‘Z’ pattern nerve endings. Arrowhead indicates GrptdTom fibers with apparent bush endings. Scale bars, 20 μm.

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