Fig. 4: Pruritogens induce Ca2+ responses in Grp+ sensory neurons. | Nature Communications

Fig. 4: Pruritogens induce Ca2+ responses in Grp+ sensory neurons.

From: Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors

Fig. 4: Pruritogens induce Ca2+ responses in Grp+ sensory neurons.The alt text for this image may have been generated using AI.

a and b Image of tdTomato neurons (a) and F340 / F380 signal (b) from GrptdTom DRG cultures loaded with fura 2-AM. Scale bar, 50 μm. c Snapshots of GrptdTom neuron intracellular Ca2+ levels at basal and 20 s after CQ (1 mM), Hist (100 μM), Cap (100 nM) or KCl (30 mM) applications. d F340/F380 traces from individual GrptdTom neurons with application of CQ, Hist, Cap and KCl. e Venn diagram of GrptdTom DRG neurons responsive to CQ, Hist or Cap and/or expressing tdTomato. n = 4 mice and 1424 neurons for a–e. Source data are provided as a Source Data file.

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