Fig. 7: Intersectional ablation of Grp spinal neurons does not affect itch or pain behavior responses. | Nature Communications

Fig. 7: Intersectional ablation of Grp spinal neurons does not affect itch or pain behavior responses.

From: Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors

Fig. 7: Intersectional ablation of Grp spinal neurons does not affect itch or pain behavior responses.The alt text for this image may have been generated using AI.

a Schematic of mating strategy of GrpCre-KI with Lbx1Flpo and Tauds-DTR lines to generate DTR-expressing Grp spinal neurons and subsequent DTX injection to ablate Grp spinal neurons. b–e ISH images of Grp expression in cervical spinal cord sections from (b, c) control and (d, e) ablated littermates. Scale bars, 100 μm in b and 20 μm in c. f–i Epifluorescent images of tdTomato in cervical spinal cord sections from (f, g) GrptdTom control and (h, i) GrptdTom ablated mice. Scale bars, 100 μm in f and 20 μm in g. j Mean number of Grp + neurons and GrptdTom neurons in control and ablated sections. n = 3 mice and 10 sections, unpaired t test, ***p < 0.001. k, l Mean number of scratches induced by i.d. nape injection of (k) CQ (100 μg) and (l) Hist (200 μg) in control and ablated mice pre- and post-DTX injection. m Mean number of wipes induced by i.d. cheek injection of Cap (20 μg) in control and ablated mice pre- and post-DTX injection. n Mean response latencies for hot plate assay at 50 °C, 52 °C, and 56 °C in control and ablated mice pre- and post-DTX injection. o Mean response latencies for Hargreaves hind-paw assay in control and ablated mice pre- and post-DTX injection. p Withdrawal thresholds (in grams of force, g) for Von Frey filament assay in control and ablated mice pre- and post-DTX injection. n = 10 mice, two-way RM ANOVA with Tukey post-hoc in k–p. Data are presented as mean ± s.e.m. Source data are provided as a Source Data file.

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