Fig. 2: Discovery and identification of shunt products accumulated in the fermentation broth of Actinoplanes sp. SE50/110. | Nature Communications

Fig. 2: Discovery and identification of shunt products accumulated in the fermentation broth of Actinoplanes sp. SE50/110.

From: A severe leakage of intermediates to shunt products in acarbose biosynthesis

Fig. 2

a HPLC profiles of the parent strain Actinoplanes sp. SE50/110 (abbreviated as SE50/110), the ∆acb mutant QQ-3 and the complemented mutant QQ-3::pLQ666. b Structures of 1-epi-valienol (8) and valienol (9). c HPLC-QQQ/MS analysis of 1 after feeding with 3 (as positive control), 8 or 9 to the ∆acbC mutant QQ-4, and fermentation of QQ-4 without feeding was set as negative control. The standard (abbreviated as std) of 1 was also analyzed. Source data underlying Fig. 2c are provided as a Source Data file.

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