Fig. 3: Identification of hydrolases involved in the dephosphorylation of 10 and 11. | Nature Communications

Fig. 3: Identification of hydrolases involved in the dephosphorylation of 10 and 11.

From: A severe leakage of intermediates to shunt products in acarbose biosynthesis

Fig. 3: Identification of hydrolases involved in the dephosphorylation of 10 and 11.The alternative text for this image may have been generated using AI.

a, b HPLC-TOF/MS analysis of the dephosphorylated products of 10 and 11 catalyzed by AcbJ, ACPL_8310, ACPL_2834, or ACPL_7709 in vitro, and the reaction without enzyme was set as a negative control. The standards (std) of 8 and 9 were also analyzed. All of the chromatograms show the simultaneous extraction of calculated ions m/z = 211.0379 [M + Cl]− for 8 and 9 and m/z = 255.0275 [M-H]− for 10 and 11. Source data are provided as a Source Data file.

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