Fig. 4: Further clarification of the C7-cyclitol moiety biosynthetic pathway by characterizing AcbL- and AcbN-catalyzed conversions. | Nature Communications

Fig. 4: Further clarification of the C7-cyclitol moiety biosynthetic pathway by characterizing AcbL- and AcbN-catalyzed conversions.

From: A severe leakage of intermediates to shunt products in acarbose biosynthesis

Fig. 4: Further clarification of the C7-cyclitol moiety biosynthetic pathway by characterizing AcbL- and AcbN-catalyzed conversions.The alternative text for this image may have been generated using AI.

a HPLC-TOF/MS analysis of the standard (std) of 12 and the reaction products of AcbL with 4, boiled AcbL with 4, AcbN with 12, boiled AcbN with 12, AcbN with 11, and boiled AcbN with 11. All the chromatograms show the extraction of corresponding calculated ions. b GC-QMS confirmation of the reaction products after dephosphorylation by AcbJ. AcbJ was added to the reaction products of AcbL & 4, boiled AcbL & 4, AcbN & 12, boiled AcbN & 12, AcbN & 11, and boiled AcbN & 11. The standards (std) 13, 8, and 9 were also analyzed by GC-QMS after derivatization by BSTFA. All the chromatograms show the extraction of corresponding unique product ions. TMS is the abbreviation of trimethyl silicyl. For more details, see also Supplementary Figs. 11, 23. Source data are provided as a Source Data file.

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