Fig. 2: BSA-PS produced via oxygen tolerant photoinduced RDRP grafting from BSA-Br. | Nature Communications

Fig. 2: BSA-PS produced via oxygen tolerant photoinduced RDRP grafting from BSA-Br.

From: Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach

Fig. 2: BSA-PS produced via oxygen tolerant photoinduced RDRP grafting from BSA-Br.The alternative text for this image may have been generated using AI.

Schematic representation of the reaction. a Top: SEC chromatographs of bioconjugates formed during monomer feed ratio optimization, with [styrene]/[BSA-Br]/[CuII]/[Me6TREN] ratios 50/1/1.5/12, 500/1/1.5/12, 2000/1/1.5/12, and 5000/1/1.5/12 (Table 1, Entries 2, 5–7); Bottom: Experimental setup and reaction syringes depicting the opaque solutions of the amphiphilic bioconjugates produced using increasing monomer concentration; b Time course of [styrene]/[BSA-Br]/[CuII]/[Me6TREN] = 2000/1/1.5/12 reaction (Table 1, Entry 5), Top: SEC traces, Bottom left: Native PAGE lane 1: 5 min, lane 2: 15 min, lane 3: 35 min, lane 4: 45 min, lane 5: 70 min, lane 6: 100 min, lane 7: 130 min, lane 8: 300 min, lane 9: native BSA, lane 10: BSA-Br (Io). Bottom right: Semiquantitative analysis plot of BSA-Br (Io) consumption during the course of the reaction; c ON/OFF time course of [styrene]/[BSA-Br]/[CuII]/[Me6TREN] = 2000/1/1.5/12 reaction (Table 1, Entry 5), Top: SEC traces, Bottom left: Native PAGE lane 1: 35 min ON, lane 2: 2 h OFF, lane 3: 35 min ON, lane 4: overnight (∼8 h) OFF, lane 5: 2 h ON, lane 6: 3 h ON, lane 7: BSA-Br. The electrophoretic gel has been cropped for clarity. Bottom right: Semiquantitative analysis plot of BSA-Br (Io) consumption during the course of the reaction.

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