Fig. 3: Using the SIMMS2 method for sequencing a hexasaccharide.
From: Shotgun ion mobility mass spectrometry sequencing of heparan sulfate saccharides
Overlaying two tetrasaccharide standard structures (#12 and #14) provides complete sequence coverage of the hexasaccharide (#16). Each structure was fragmented in the trap of the mass spectrometer and analyzed using drift tube ion mobility mass spectrometry (DTIMS), resulting in accurate collision cross section (CCS) values. A comparison of overlapping fragment ions displayed the same CCS value between the two tetra- and hexasaccharides, providing a three-dimensional (MS, MS/MS and IMMS) sequencing method. a–f The hexasaccharide displays the DTIMS data to be determined. g, h Tetrasaccharide #12 displayed CCS values from B ions; B2—135 Å2 and B3—160 Å2 matched CCS values observed in the hexasaccharide (a–c). i, j Tetrasaccharide #14 displayed CCS values from Y ions; Y2 156 Å2 and Y3 213 Å2 matched the CCS values observed in the hexasaccharide (d–f).
