Fig. 2: TEAD transcriptional networks in keratinocytes regulate cell cycle entry and differentiation programs. | Nature Communications

Fig. 2: TEAD transcriptional networks in keratinocytes regulate cell cycle entry and differentiation programs.

From: YAP1/TAZ-TEAD transcriptional networks maintain skin homeostasis by regulating cell proliferation and limiting KLF4 activity

Fig. 2

a Western blot analysis of expression of GFP (Ad-GFP, control) and TEADi (Ad-TEADi) in N/TERT2G cells. b Venn diagram showing the overlap between differentially regulated genes in TEADi vs GFP (q < 0.05, |FC| ≥ 1.5) in N/TERT2G keratinocytes at 24 and 48 h following transduction. c, d Venn diagrams showing the overlap between differentially regulated genes (q < 0.05, |FC| ≥ 1.5) by TEADi expression in N/TERT2G keratinocytes and published YAP1 signatures13,26 (c) or siYAP1/TAZ (d). e Graph indicating the fold change (Log2 FC) of genes related to canonical Wnt/β-catenin signaling (IPA) in the siYAP1/TAZ and TEADi 48 h datasets. Only differentially regulated genes are shown. White color indicates the gene is not differentially regulated in that dataset. The IPA significance p-value (p) of overlap for the Wnt/β-catenin canonical pathway is indicated for each dataset. f IPA activation Z-score for canonical Wnt/β-catenin signaling in the indicated datasets. g Western blot analysis of expression of the indicated markers in N/TERT2G cells. Active β-Catenin refers to non-phospho β-Catenin at Ser45. h Graph indicating the GO biological process terms enriched in upregulated (q < 0.05, FC ≥ 1.5) and downregulated (q < 0.05, FC ≤ −1.5) genes by TEADi expression in N/TERT2G keratinocytes at 48 h and fold change (Log2 FC) of genes related to GO keratinocyte differentiation and cell cycle at corresponding times compared with GFP expressing cells. i IPA functional analysis of activation state of upstream transcriptional regulators for genes in b. j Western blot analysis of the expression of cell cycle regulators in N/TERT2G cells transduced with GFP (Ad-GFP, control) or TEADi (Ad-TEADi) for 48 h. k Luminescence cell proliferation assay in N/TERT2G cells transduced with pooled siRNAs for E2F1. l Western blot analysis of the expression of cell cycle regulators and differentiation markers in N/TERT2G cells transduced with pooled siRNAs for E2F1 for 48 h. In k n = 3 biological replicates. Mean ± SD is shown; two-way ANOVA with Sidak’s multiple comparison test. In b, c and d p indicates the p-value of the overlap in Venn diagrams, Fisher’s exact test. Source data are provided as a Source Data file.

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