Fig. 5: TEAD blockage in the mouse skin induces keratinocyte differentiation and progenitor cell depletion. | Nature Communications

Fig. 5: TEAD blockage in the mouse skin induces keratinocyte differentiation and progenitor cell depletion.

From: YAP1/TAZ-TEAD transcriptional networks maintain skin homeostasis by regulating cell proliferation and limiting KLF4 activity

Fig. 5

a Schematic representation of the animal model used to target the inducible expression of the TEADi to the basal epidermal stem cell compartment. b Histological analysis of wild type (WT) mice and mice induced to express TEADi for 4 and 12 days and IF showing nuclear expression of TEADi. c–f Representative IF pictures of the indicated markers and corresponding expression quantification from the skin of WT or TEADi mice induced for 4 or 12 days. Inserts show magnification of highlighted area and location of the basal membrane is indicated with a white dotted line. In d and f the different fluorescent channels of the insert magnification are presented individually bellow the picture for better clarity. In c WT n = 10, TEADi-4d n = 11, TEADi-12d n = 16; (d) WT n = 14, TEADi-4d n = 18, TEADi-12d n = 19; (e) WT n = 14, TEADi-4d n = 15, TEADi-12d n = 16; and f WT n = 14, TEADi-4d n = 18, TEADi-12d n = 19 fields from 3 different mice in each condition. Violin plots shows density of data and box plot shows median, 25 and 75% quartile, 95% confidence interval and dots indicating potential outliers; two-way ANOVA with Dunnett’s multiple comparison test. Source data are provided as a Source Data file.

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