Fig. 4: Rapid library generation and quantitation of Plasmodium falciparum proteins.
From: Generating high quality libraries for DIA MS with empirically corrected peptide predictions
a A proportionally sized Venn diagram showing the overlap between proteins detected in the empirically corrected library (blue), the full single-injection dilution curve with DDA (red), and the Stage IV/V mixed male/female sexual P. falciparum proteome published by Lasonder et al.31 from 20× GeLCMS-fractionated DDA acquisitions (yellow). Quantitative ratios of b 2740 DIA- and c 1491 DDA-detected malaria parasite P. falciparum proteins (gray lines) after dilution with uninfected RBC lysate relative to the undiluted sample (N = 1). Dots indicate the lowest dilution level of measurement for each protein, and the blue dashed line indicates the ideal measurement. Empirically corrected libraries enabled DIA quantification of 2152 P. falciparum proteins in the last dilution sample (1:99), even when no proteins could be detected or quantified using DDA. Source data are provided as a Source Data file.
