Fig. 1: HY5 interacts with BIN2.
From: Modulation of BIN2 kinase activity by HY5 controls hypocotyl elongation in the light
a HY5 interacts with BIN2 in yeast. Full-length HY5 was fused with AD. b Schematic representation of HY5. HY5-F, full-length HY5; HY5-N, aa 1–77; HY5-C, aa 78–168; HY5-ΔLZ, HY5 lacking leucine zipper region (LZ, aa 115–147). c In vitro pull-down assay showing the interaction between HY5 and BIN2. Full-length and truncated HY5 were fused with the GST-tag; BIN2 was fused with the His-tag. Asterisk indicates a non-specific band. d LCI assay showing the interaction between HY5 and BIN2 in N. tabacum leaves. nLUC, the vector containing the N-terminal fragment of firefly luciferase. cLUC, the vector containing the C-terminal fragment of firefly luciferase. Full-length and truncated HY5 was fused with nLUC and BIN2 was fused with cLUC. The N. tabacum leaves were infiltrated with the indicated combinations. cps, counts per second. e–g Co-IP assays showing HY5 associated with BIN2. HSP was used as a loading control. Source data are provided as a source data file.
