Fig. 2: HY5 genetically interacts with BIN2 and BZR1 to inhibit hypocotyl elongation in the light.
From: Modulation of BIN2 kinase activity by HY5 controls hypocotyl elongation in the light
a Hypocotyl lengths of Col, bin2-1, hy5, and hy5bin2-1 mutant seedlings. b Hypocotyl lengths of lines overexpressing HY5 in Ws and bin2-3bil1bil2 backgrounds. bin2-3bil1bil2, null mutant of BIN2 and its closest homologs, BIL1 and BIL2. HA-HY5, transgenic seedlings overexpressing 3Ă—HA-tagged HY5. c Hypocotyl lengths of HA-HY5 line seedlings treated with 30 μM bikinin (BK), an inhibitor of Arabidopsis GSK3-like kinases. d Hypocotyl lengths of light-grown seedlings of hy5bzr1bes1 and bzr1bes1. BZR1 and BES1 double mutants were generated by CRISPR/Cas9. Seedlings were grown for 6 days under continuous white light (cWL). Seedlings were grown under 20 W m−2 (a, d) or 10 W m−2 (b, c) light intensity, respectively. Error bars represent standard deviation (SD), n ≥ 20. Asterisks indicate the P value of two-tailed Student’s t-test; ***P < 0.001. Experiments were performed three times with similar results. Bar = 1 mm. Source data are provided as a source data file.
