Fig. 2: Targeting MAO-A resensitizes EnzR cells to Enz and suppresses EnzR cell growth.

a EnzS1-C4-2 cells were treated with/without (w/o) 10 μM Enz and 5 μM clorgyline, and cell viability analyzed by MTT assay; (n = 3 biological independent samples); p = 0.03. b–d EnzR1-C4-2, EnzR2-C4-2, and EnzR3-22Rv1 cells, respectively, were treated (w/o) 10 μM Enz and 5 μM clorgyline, and cell viability was analyzed by MTT assay; (n = 3 biological independent samples); p = 0.02 for b, p = 0.03 for c, and p = 0.03 for d. e–g EnzR1-C4-2, EnzR2-C4-2, and EnzR3-22Rv1 cells, respectively, were treated w/o 10 μM Enz and 5 μM phenelzine, and cell viability was analyzed; (n = 3 biological independent samples); p = 0.03 for e, p = 0.04 for f, and p = 0.03 for g. h–j The EnzR1-C4-2, EnzR2-C4-2, and EnzR3-22Rv1 cells with pLKO or shMAO-A cells were treated w/o Enz and cell viability was analyzed; (n = 3 biological independent samples); p = 0.02 for h, p = 0.02 for i, and p = 0.01 for j. k MAO-A was overexpressed (oeMAO-A) in EnzS1-C4-2 cells and then the cells treated w/o Enz and cell viability was analyzed; (n = 3 biological independent samples); p = 0.002 for k. l EnzS1-C4-2 cells were treated w/o 10 μM Enz and cell viability was analyzed; (n = 3 biological independent samples); p = 0.001. m EnzS1-C4-2 cells were first treated (w/o) 10 μM Enz for 1.5 months. And then the cells were seeding and the cell viability under 10 μM Enz treatment was analyzed by MTT assay; (n = 3 biological independent samples); p = 0.02. n, o EnzS1-C4-2 cells were treated w/o 10 μM Enz and 1 μM (n) or 2.5 μM (o) clorgyline (clg) for 1.5 months. And then the cell viability under 10 μM Enz treatment was analyzed; (n = 3 biological independent samples); p = 0.01 for n and p = 0.01 for o. Data represent the mean ± SEM, error bars represent SEM. p-value was determined by two-tailed paired t-test.