Fig. 3: Mechanism dissection of how Enz increases the MAO-A expression: via increasing ARv7.

a, b PC3 cells were treated with/without (w/o) 10 μM Enz and 5 μM clorgyline or 10 μM clorgyline. The cell viability was analyzed by MTT assay; (n = 3 biological independent samples). c EnzS1-C4-2 cells were treated with 10 μM Enz for different time points. The ARv7 and MAO-A mRNA level were analyzed by qPCR (left) and western blot (right); (n = 3 biological independent samples). d The mRNA levels of ARv7 and MAO-A were analyzed in EnzR1-C4-2 pWPI and pWPI-ARv7 cells. e The expression of ARv7 and MAO-A were analyzed in EnzS1-C4-2 pWPI and pWPI-ARv7 (oeARv7) cells by qPCR (left) and western blot (right). f–h The mRNA and protein level of ARv7 and MAO-A were analyzed in EnzR1-C4-2, EnzR2-C4-2 (no protein shown), EnzR3-22Rv1 pLKO, and shARv7 cells; (n = 3 biological independent samples for qPCR). i EnzS1-C4-2 cells were infected with pLKO and shARv7 viruses. And then the cells were treated w/o Enz for 2 and 6 days, the ARv7 and MAO-A levels were examined by qPCR; (n = 3 biological independent samples). j EnzS1 cells were infected by pWPI-ARv7, pLKO-shMAO-A, or both viruses. And then the cells were treated w/o 10 μM Enz and the cell viability analyzed by MTT assay; (n = 3 biological independent samples). For c and f–i, data represent the mean ± SEM, error bars represent SEM. p-value was determined by two-tailed paired t-test.