Fig. 4: RBRP interacts with the m6A reader IGF2BP1.
From: An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis
a Proteins that interacted with RBRP were identified by co-IP together with mass spectrometry. b, c The LINC00266-1 ORF-Flag (upper panel) and IGF2BP1-HA (lower panel) vectors were transfected into HEK293T cells, RBRP-Flag and IGF2BP1-HA complexes were co-IPed with anti-Flag and anti-HA antibodies in the absence (b) or presence (c) of RNase A treatment, and IGF2BP1 and RBRP/HA were detected, respectively. d Diagram of the different domains of the WT and mutated-IGF2BP1 constructs. e, f The indicated IGF2BP1-HA mutants were cotransfected with the LINC00266-1 ORF-Flag vector into HEK293T cells; RBRP-Flag (e) and IGF2BP1-HA (f) complexes were co-IPed with anti-Flag and anti-HA antibodies, respectively. The IGF2BP1-HA mutant and RBRP-Flag complexes were detected using anti-HA and anti-Flag antibodies, respectively. g, h The WT and GxxGΔ-mutated IGF2BP1-HA vectors were transfected into HEK293T cells and the interactions of RBRP with the IGF2BP1 GxxGΔ mutant were determined. i, j The WT or mutated RBRP-Flag constructs were cotransfected with the IGF2BP1-HA plasmid into HEK293T cells, and the interactions of the RBRP mutants with IGF2BP1 were determined. Source data are provided as a Source Data file.
