Fig. 4: Burst analysis of Ang II-elicited calcium spikes.
From: Angiotensin II induces coordinated calcium bursts in aldosterone-producing adrenal rosettes
Burst duration and number of spikes/burst are consistent across Ang II concentrations. a Box plot of individual burst duration, median value (25–75%): 50 pM: 14.6 (8.2–14.6); 300 pM: 13.9 (7.3–29.3); 3 nM: 13.9 (7.3-30.8); 1 µM: 11.2 (6.3–26.0); b means ± SEM [s]: 50pM: 26.3 ± 2.2; 300 pM: 26.3 ± 2.2; 3 nM: 29.4 ± 2.7; 1 µM: 23.6 ± 2.1; Kruskal–Wallis test: P = 0.4783; c box plot of individual burst spikes, median value (25–75%): 50 pM: 6 (4–14), 300 pM: 6 (4–14), 3 nM: 7 (4–14.5), 1 µM: 9 (5–16); d means ± SEM; Kruskal–Wallis test: P = 0.65). e However, both the time it took for a cell to respond to Ang II (time to first burst, means ± SEM (s): 50 pM: 291 ± 44; 300 pM: 252 ± 11;3 nM: 79 ± 9; 1 µM: 81 ± 21; Kruskal–Wallis test: P = 0.002, Dunn’s multiple comparison test: 50 pM vs 3 nM: P = 0.006; 50 pM vs 1 µM P = 0.0398; non-linear curve fit: R2 = 0.77, IC50 = 366.1 pM) and fraction of nonburst spikes (f, g) decreased with Ang II dose (f distribution of the fraction of non-burst spikes in individual cells, 50 pM: n = 74; 300 pM: n = 250; 3 nM: n = 151; 1 µM: n = 95; g means ± SEM: 50 pM: 0.32 ± 0.05; 300 pM: 0.19 ± 0.03; 3 nM: 0.13 ± 0.02; 1 µM: 0.12 ± 0.01; Kruskal–Wallis test, P = 0.008, Dunn’s multiple comparison test: 50 pM vs 3 nM P = 0.0078, non-linear curve fit: R2 = 0.77, IC50 = 366.1 pM). Unless otherwise noted, for Ang II concentrations: 50 pM: n = 5, 135 cells; 300 pM: n = 6, 1017 cells; 3 nM: n = 7, 1398 cells; 1 µM: n = 4, 1098 cells. b, d, e, g Mean data from each mouse were represented as a single point in calculating N/experimental condition. Bars and symbols are color coded according to experimental condition; 50 pM: black, 300 pM Ang II: blue, 3 nM Ang II: orange, 1 µM Ang II: red. Source data are provided as a Source Data file.
