Fig. 6: Sequence alignment of AEPs. Proposed ligase-activity determinant (LAD) residues are boxed in orange. | Nature Communications

Fig. 6: Sequence alignment of AEPs. Proposed ligase-activity determinant (LAD) residues are boxed in orange.

From: A bifunctional asparaginyl endopeptidase efficiently catalyzes both cleavage and cyclization of cyclic trypsin inhibitors

Fig. 6: Sequence alignment of AEPs. Proposed ligase-activity determinant (LAD) residues are boxed in orange.

Residues from LAD2 or LAD1 gatekeeper (GK) are in red (ligase favored) and green (protease favored), respectively. The poly-proline loop (PPL) is demarcated by a blue box and the region defined as a marker of ligase activity (MLA) is boxed in magenta. AEP activity was either predicted based on sequence similarity or experimentally verified in vitro and/or in planta.

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