Table 1 Peptide substrates used in this study.

From: A bifunctional asparaginyl endopeptidase efficiently catalyzes both cleavage and cyclization of cyclic trypsin inhibitors

Peptide

Sequence

obs. Massa

calc. Massa

MCoTI-II-DAL

GGV-CPKILKKCRRDSDCPGA-CICRGNGYCGSGSDAL

3653.69

3653.67

MCoTI-II-NAL

GGV-CPKILKKCRRDSDCPGA-CICRGNGYCGSGSNAL

3652.91

3652.70

DIN-MCoTI-II

DINGGV-CPKILKKCRRDSDCPGA-CICRGNGYCGSGSD

3811.76

3811.71

DIN-MCoTI-II-DAL

DINGGV-CPKILKKCRRDSDCPGA-CICRGNGYCGSGSDAL

3995.93

3995.83

DID-MCoTI-II-NAL

DIDGGV-CPKILKKCRRDSDCPGA-CICRGNGYCGSGSNAL

3995.92

3995.83

AcDID-MCoTI-II-NAL

DIDGGV-CPKILKKCRRDSDCPGA-CICRGNGYCGSGSNALb

4037.89

4037.83

TIPTOP2 unit 3

…GGV-CPKILKKCRRDSDCPGA-CICRGNGYCGSGSD…c

6867.20

6867.21

MCoSST-01-DAL

GGV-CPKILKKCRRDSDCPGA-CICRYwKVCGSGSDAL

3838.90

3838.83

kB1-NAL

GLPVCGET—CVGGT-CNTPGCTCSWPV-CTR–NAL

3093.34

3093.32

  1. Sequences in bold denote the truncated leader peptide and truncated follower peptide. P1 Asx residues are underlined. MCoSST-01-DAL is a grafted peptide based on the scaffold of wild type MCoTI-II, and the italicized residues correspond to the grafted epitope. Lower case w indicates D-Trp
  2. aObserved (obs.) and calculated (cal.) monoisotopic mass [M + H]+
  3. bN-terminal residue is acetylated
  4. cThis peptide carries the full-length leader (ALEGLMSDSRAQIDIN) and follower (ALEGLMSDGRAQIDIN) sequences at its N and C termini, respectively
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